Integral membrane TGN38A (350-361) acetyl, mouse – Other Categories

Integral membrane protein TGN38 cycles via endosomes between the cell surface and the trans-Golgi network (TGN). Peptides conjugated to the TGN38 sequence inhibit vesicle budding from TGN membranes-in vitro.-This leads to the conclusion that TGN38 is crucial for forming constitutive exocytic vesicles. The trafficking motif SDYQRL in the cytoplasmic domain at the C-terminal contains a critical tyrosine residue (Y). The motif interacts with the medium-chain adaptor subunits of AP-1/2 to allow sorting from the TGN. Mutation of the key tyrosine dramatically reduces binding affinity to AP-1/2. The flanking sequences to the trafficking motif affect the TGN38 localisation of hybrid proteins. Working with the mouse allele TGN38a has helped understand the conservation between mammalian, rat, and mouse TGN protein sequence and function. The TGN38a trafficking motif with flanking regions is provided here in an acid form with N-terminal acetylation.