HiBiT tag – Other Categories

NanoLuc (Nluc) is an engineered luciferase protein which was developed from the luciferase of deep-sea shrimp (Oplophorus gracilirostris). This luciferase protein is considerably smaller than firefly or Renilla luciferase yet has higher luminescent intensity. In the NanoBiT assay system the NanoLuc luciferase protein has been separated into a large fragment, LgBiT, and a small fragment. HiBiT has a very similar amino acid sequence to the original small fragment and therefore has high specific affinity for the N-terminal large fragment, LgBiT. When these two fragments interact NanoLuc activity is restored. This system offers a novel alternative to conventional immunoblot analysis for the detection of protein expression when the HiBiT tag is added to the protein of interest and cell lysate is incubated with LgBiT. HiBiT peptide is capable of producing bright and quantitative luminescence through high affinity complementation with an 18 kDa subunit derived from NanoLuc (LgBiT).