[DABCYL]/[Glu(EDANS)] SARS-CoV-2 3C-like protease (3CLpro) substrate – Fluorescent Peptides

3CLpro are the key enzymes required by coronaviruses to replicate. They cleave polyproteins to form replicase. This makes 3CLpro a drug target for protease inhibitors with particular interest to COVID-19. Synthetic 3CLpro substrates are being generated for their potential to inhibit the protease activity and thus replication cycle of coronaviruses. When this peptide is intact, fluorescence from the fluorophore (donor) EDAN is undetectable due to the proximity of the acceptor (quencher) Dabcyl. However, upon cleavage the fluorescence of the EDANS moiety, as measurably by excitation/emission 340/490nm, can be detected due to separation from the Dabcyl quencher. This product was shown to be a potent inhibitor of 3CLpro activity therefore, it has the potential to be a vital tool in the fight against SARS-CoV.

Technical specification

Price