Cathepsin G FRET substrate [5-FAM]/[6-TAMRA] – Fluorescent Peptides

Substrate peptide for Cathepsin G, a serine protease belonging to the chymotrypsin superfamily which acts as a physiologic regulator of platelet activation and thrombus formation. Cathepsin G also has antimicrobial activity and is involved in chemotaxis, apoptosis, the immune response and inflammation and hydrolysis of extracellular matrix proteins. Cathepsin G can cleave protease activated receptor-4 (PAR4) and is a potential target for novel anti-thrombotic therapies. This peptide contains the FRET pair: 5-Carboxyfluorescein (5-FAM) and 6-Carboxytetramethylrhodamine (6-TAMRA). 5-Carboxyfluorescein (5-FAM), which is present at the N-terminus of this peptide, is a widely used green fluorescent tag which excites at 495 nm and emits at 517 nm. 6-carboxytetramethylrhodamine (6-TAMRA), which is attached at the C-terminus, is a widely used fluorescent dye which excites at 546 nm and emits at 579 nm. In the intact peptide 6-TAMRA acts as a quencher of the fluorescence of 5-FAM therefore no fluorescence can be detected. However hydrolysis of the peptide between the donor/acceptor pair allows the fluorescence of 5-FAM to be recovered, enabling the quantitative measurement of enzymatic activity. Fluorescence Resonance Energy Transfer (FRET) peptides are convenient tools for the study of peptidase specificity and enzymatic activity.

Technical specification

Price