Peptide purification method development

Peptide purification is generally the most fastidious and expensive step of peptide production. Peptides are amphipathic heteropolymers and each peptide possesses specific characteristics in terms of hydrophobicity, hydrophilicity and charges. Therefore, a specific purification method for each peptide need to be developed in order to maximize the purification efficiency. For that reason, it is very important to well integrate the purification method development in the global process of production.

Reversed phase chromatography represents the state-of-the-art technique for the peptide purification process but some other techniques, depending on peptides properties (hydrophobicity, hydrophilicity…), may exist in order to improve peptide purity rate. In some cases, orthogonal method may be used to achieve the purity rate desired. Many parameters can be tuned to obtain best results such as mode (C18, C12, C3, IEX, mix mode…), gradient, temperature, pH, buffer, particule size… 

A key step in the peptide purification method development is the identification of critical impurities. Critical impurities are those closely eluting with the target product whom are by consequent hard to eliminate. Optimizing peptide purification method to properly separate the target peptides from close purities is a major step in the process development.

sb-PEPTIDE is using a screening panel of different chromatography media and other tunable parameters to obtain best results. sb-PEPTIDE can manage global process method development or only synthesis or purification method development separately.

Developed methods can then be transfered to clients or subcontractors.

Contact us for discussing about the project.